细胞生长:贴壁生长 细胞形态:上皮细胞样 细胞数量:1×106个 细胞传代:1:6~1:10传代;每周换液1~2次 细胞特性:Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance). The line shows characteristics of a stage between type B spermatogonia and primary spermatocytes. The cells express two testis specific isoproteins, cytochrome c and lactate dehydrogenase C4. 细胞纯度:93% 细胞活力:87%(Viability by Trypan Blue Exclusion) 细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌 细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS) 细胞运输:干冰运输(1 Vial)或活细胞运输(T-25 flasks) 细胞用途:只可用于科研,不可用于临床诊断和治疗